Lab protocols

A collection of recipes and methods that surprisingly have worked!

Please feel free to copy and distribute. If you contact us we can provide more details of these methods and references where they were published. Thanks!

A collection of general use buffers

Preparation of fixed erythrocytes to reveal ECM in cultured cells

Preparation of brain slices

Measure of TACE/ADAM17 activity

Cell adhesion assay

Immunocytochemistry

Fluorescence immunohistochemistry

Culturing cells on nanofiber scaffolds

Simple immunoprecipitation procedure

Protocols for protein deglycosylation

Antibody coupling to nanoparticles

Myelin preparation from adult brain

Nissl staining of brain tissue sections

Silver staining of SDS-PAGE gels

Classical subcellular fractionation for brain tissue

A primer for stereotaxic surgery in adult rats